Review



human hcc tissue microarray tma  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Novus Biologicals human hcc tissue microarray tma
    Identification of downstream target genes commonly upregulated by mitochondrial defect and NFE2L1. Transcriptome data of four cell lines (Ch-L and SNU387 harboring active mitochondria, and SNU354 and SNU423 harboring defective mitochondria) were obtained from our previous report . Two independent sets of SNU387 cells transfected with pcDNA-NFE2L1 plasmid (NFE2L1_OE) for 48 h and of SNU423 cells with siNFE2L1 (NFE2L1_KD) for 72 h were applied to cDNA <t>microarray</t> for transcriptome profiling. ( A ) Venn diagram of commonly upregulated genes with mitochondrial defect (Mito defect, SNU354 and SNU423 vs. Ch-L and SNU387) and NFE2L1-dependent regulation. ( B ) Heatmaps show the expression of 10 commonly upregulated genes in four hepatoma cell lines (left), NFE2L1-depleted SNU423 cells (middle), and NFE2L1-overexpressed SNU387 cells (right). Each column and row represent independent samples and the indicated genes, respectively. Red and blue color indicates the high and low expression, respectively. ( C , E ) Validation of four target mRNA levels by qRT-PCR. ( D , F ) Validation of four target protein levels by Western blot analysis. #1 and #2 indicate two different siRNAs for NFE2L1. ** p < 0.01 vs. pcDNA or siNC by the Student t -test. ( G ) Associations of NFE2L1 with the 10 commonly upregulated genes were evaluated using the TCGA-LIHC cohort ( n = 371). Pearson’s product moment correlation test was performed. The correlation estimate and the p-value with statistical significance are marked. ( H ) Boxplot for STX12 expression level of primary <t>HCC</t> tissues from the TCGA-LIHC cohort. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. For comparison, primary tumors were divided into two groups, based on the median expression level of NFE2L1 and NDUFA9: a group with both above the median NFE2L1 level and below the median NDUFA9 level, a group with both below the median NFE2L1 level and above the median NDUFA9 level. p -value from Wilcoxon t-test is indicated.
    Human Hcc Tissue Microarray Tma, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/Human+Liver+Tissue+MicroArray+(Cancer+metastasis)/pmc07565734-219-5-11
    Average 90 stars, based on 13 article reviews
    human hcc tissue microarray tma - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis"

    Article Title: Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis

    Journal: Cancers

    doi: 10.3390/cancers12092632

    Identification of downstream target genes commonly upregulated by mitochondrial defect and NFE2L1. Transcriptome data of four cell lines (Ch-L and SNU387 harboring active mitochondria, and SNU354 and SNU423 harboring defective mitochondria) were obtained from our previous report . Two independent sets of SNU387 cells transfected with pcDNA-NFE2L1 plasmid (NFE2L1_OE) for 48 h and of SNU423 cells with siNFE2L1 (NFE2L1_KD) for 72 h were applied to cDNA microarray for transcriptome profiling. ( A ) Venn diagram of commonly upregulated genes with mitochondrial defect (Mito defect, SNU354 and SNU423 vs. Ch-L and SNU387) and NFE2L1-dependent regulation. ( B ) Heatmaps show the expression of 10 commonly upregulated genes in four hepatoma cell lines (left), NFE2L1-depleted SNU423 cells (middle), and NFE2L1-overexpressed SNU387 cells (right). Each column and row represent independent samples and the indicated genes, respectively. Red and blue color indicates the high and low expression, respectively. ( C , E ) Validation of four target mRNA levels by qRT-PCR. ( D , F ) Validation of four target protein levels by Western blot analysis. #1 and #2 indicate two different siRNAs for NFE2L1. ** p < 0.01 vs. pcDNA or siNC by the Student t -test. ( G ) Associations of NFE2L1 with the 10 commonly upregulated genes were evaluated using the TCGA-LIHC cohort ( n = 371). Pearson’s product moment correlation test was performed. The correlation estimate and the p-value with statistical significance are marked. ( H ) Boxplot for STX12 expression level of primary HCC tissues from the TCGA-LIHC cohort. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. For comparison, primary tumors were divided into two groups, based on the median expression level of NFE2L1 and NDUFA9: a group with both above the median NFE2L1 level and below the median NDUFA9 level, a group with both below the median NFE2L1 level and above the median NDUFA9 level. p -value from Wilcoxon t-test is indicated.
    Figure Legend Snippet: Identification of downstream target genes commonly upregulated by mitochondrial defect and NFE2L1. Transcriptome data of four cell lines (Ch-L and SNU387 harboring active mitochondria, and SNU354 and SNU423 harboring defective mitochondria) were obtained from our previous report . Two independent sets of SNU387 cells transfected with pcDNA-NFE2L1 plasmid (NFE2L1_OE) for 48 h and of SNU423 cells with siNFE2L1 (NFE2L1_KD) for 72 h were applied to cDNA microarray for transcriptome profiling. ( A ) Venn diagram of commonly upregulated genes with mitochondrial defect (Mito defect, SNU354 and SNU423 vs. Ch-L and SNU387) and NFE2L1-dependent regulation. ( B ) Heatmaps show the expression of 10 commonly upregulated genes in four hepatoma cell lines (left), NFE2L1-depleted SNU423 cells (middle), and NFE2L1-overexpressed SNU387 cells (right). Each column and row represent independent samples and the indicated genes, respectively. Red and blue color indicates the high and low expression, respectively. ( C , E ) Validation of four target mRNA levels by qRT-PCR. ( D , F ) Validation of four target protein levels by Western blot analysis. #1 and #2 indicate two different siRNAs for NFE2L1. ** p < 0.01 vs. pcDNA or siNC by the Student t -test. ( G ) Associations of NFE2L1 with the 10 commonly upregulated genes were evaluated using the TCGA-LIHC cohort ( n = 371). Pearson’s product moment correlation test was performed. The correlation estimate and the p-value with statistical significance are marked. ( H ) Boxplot for STX12 expression level of primary HCC tissues from the TCGA-LIHC cohort. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. For comparison, primary tumors were divided into two groups, based on the median expression level of NFE2L1 and NDUFA9: a group with both above the median NFE2L1 level and below the median NDUFA9 level, a group with both below the median NFE2L1 level and above the median NDUFA9 level. p -value from Wilcoxon t-test is indicated.

    Techniques Used: Transfection, Plasmid Preparation, Microarray, Expressing, Biomarker Discovery, Quantitative RT-PCR, Western Blot, Comparison

    STX12 is a key regulator of hepatoma cell invasiveness. ( A , B ) SNU423 cell was transfected with STX12 siRNA for 72 h. ( A ) Cell invasion assay using a Matrigel-coated Transwell system. Representative invaded cell images are shown in the right panel. ** p < 0.01 vs. siNC by the Student t-test. ( B ) Western blot (left) and cell growth rate (right). #1 and #2 indicate two different siRNAs for STX12. ( C , D ) Ch-L was transfected with STX12 plasmid for 48 h. ( C ) Cell invasion assay. Representative invaded cell images are shown in the right panel. ** p < 0.01 vs. GFP by the Student t-test. ( D ) Western blot (left) and cell growth rate (right). ( E – G ) SNU423 clone, which was stably suppressing NFE2L1 with sgNFE2L1 RNA, was further infected with lentivirus harboring STX12 or GFP. ( E ) Cell invasion assay. ** p < 0.01 vs. sgNC; # < 0.05 vs. GFP by the Student t -test. ( F ) Cell growth. ( G ) Western blot. ( H ) Immunohistochemistry of HCC tissue microarray, as described in ‘Materials and Methods’. Lower panel shows representative images for NFE2L1 and STX12 immunostaining. ( I , J ) Boxplots of the normalized enrichment score (NES) for EMT signature of the primary HCC tissues from the TCGA_LIHC dataset. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. p -values from Welch two-sample t -test are indicated. ( I ) Tumor samples were divided into high and low groups, according to the expression level of NFE2L1 (left) or STX12 (middle), respectively, as described in ‘Materials and Methods’. To evaluate the effect of co-expression on the EMT signature, tumor samples were divided into two groups, based on the co-expression level of both NFE2L1 and STX12 (right). ( J ) The NFE2L1 and STX12 co-expressing group (right panel of I) was further subdivided into an H ( n = 11) and L group ( n = 23) according to the NDUFA9 expression level. ( K ) Overall survival (OS) time of the H and L group was compared based on the Kaplan–Meier survival analysis. Statistical significance for KM survival was estimated by the Cox–Mantel log-rank test.
    Figure Legend Snippet: STX12 is a key regulator of hepatoma cell invasiveness. ( A , B ) SNU423 cell was transfected with STX12 siRNA for 72 h. ( A ) Cell invasion assay using a Matrigel-coated Transwell system. Representative invaded cell images are shown in the right panel. ** p < 0.01 vs. siNC by the Student t-test. ( B ) Western blot (left) and cell growth rate (right). #1 and #2 indicate two different siRNAs for STX12. ( C , D ) Ch-L was transfected with STX12 plasmid for 48 h. ( C ) Cell invasion assay. Representative invaded cell images are shown in the right panel. ** p < 0.01 vs. GFP by the Student t-test. ( D ) Western blot (left) and cell growth rate (right). ( E – G ) SNU423 clone, which was stably suppressing NFE2L1 with sgNFE2L1 RNA, was further infected with lentivirus harboring STX12 or GFP. ( E ) Cell invasion assay. ** p < 0.01 vs. sgNC; # < 0.05 vs. GFP by the Student t -test. ( F ) Cell growth. ( G ) Western blot. ( H ) Immunohistochemistry of HCC tissue microarray, as described in ‘Materials and Methods’. Lower panel shows representative images for NFE2L1 and STX12 immunostaining. ( I , J ) Boxplots of the normalized enrichment score (NES) for EMT signature of the primary HCC tissues from the TCGA_LIHC dataset. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. p -values from Welch two-sample t -test are indicated. ( I ) Tumor samples were divided into high and low groups, according to the expression level of NFE2L1 (left) or STX12 (middle), respectively, as described in ‘Materials and Methods’. To evaluate the effect of co-expression on the EMT signature, tumor samples were divided into two groups, based on the co-expression level of both NFE2L1 and STX12 (right). ( J ) The NFE2L1 and STX12 co-expressing group (right panel of I) was further subdivided into an H ( n = 11) and L group ( n = 23) according to the NDUFA9 expression level. ( K ) Overall survival (OS) time of the H and L group was compared based on the Kaplan–Meier survival analysis. Statistical significance for KM survival was estimated by the Cox–Mantel log-rank test.

    Techniques Used: Transfection, Invasion Assay, Western Blot, Plasmid Preparation, Stable Transfection, Infection, Immunohistochemistry, Microarray, Immunostaining, Expressing

    Related Articles

    Immunohistochemistry:

    Article Title: Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC)
    Article Snippet: .. Immunohistochemistry and Western Blotting Immunohistochemistry was performed in human HCC tissue microarrays containing 40 primary HCC, 10 metastatic HCC, and 9 normal adjacent liver samples (Imgenex; IMH-360) ( 7 ). .. The primary antibodies used were as follows: DIO1 (ProteinTech; rabbit polyclonal; 1:100) and AEG-1 (chicken polyclonal; 1:500).

    Article Title: Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC)
    Article Snippet: .. Immunohistochemistry was performed in human HCC tissue microarrays containing 40 primary HCC, 10 metastatic HCC, and 9 normal adjacent liver samples (Imgenex; IMH-360) ( 7 ). .. The primary antibodies used were as follows: DIO1 (ProteinTech; rabbit polyclonal; 1:100) and AEG-1 (chicken polyclonal; 1:500).

    Western Blot:

    Article Title: Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC)
    Article Snippet: .. Immunohistochemistry and Western Blotting Immunohistochemistry was performed in human HCC tissue microarrays containing 40 primary HCC, 10 metastatic HCC, and 9 normal adjacent liver samples (Imgenex; IMH-360) ( 7 ). .. The primary antibodies used were as follows: DIO1 (ProteinTech; rabbit polyclonal; 1:100) and AEG-1 (chicken polyclonal; 1:500).

    Microarray:

    Article Title: Staphylococcal Nuclease and Tudor Domain Containing 1 (SND1 Protein) Promotes Hepatocarcinogenesis by Inhibiting Monoglyceride Lipase (MGLL)
    Article Snippet: .. Tissue Microarray and Immunostaining Human HCC tissue microarrays were obtained from Imgenex Corp. Two tissue microarrays were used as follows: one containing 40 primary HCC, 10 metastatic HCC, and 9 normal adjacent liver samples (Imgenex; IMH-360); and the other containing 46 primary HCC and 13 metastatic HCC (Imgenex; IMH-318). .. Immunostaining was performed using anti-SND1 antibody (rabbit polyclonal; 1:100; Prestige Antibodies® powered by Atlas antibodies from Sigma), anti-MGLL antibody (goat polyclonal; 1:250; Abcam), and anti-PCNA antibody (mouse monoclonal; 1:300; Cell Signaling) as described ( 16 ).

    Immunostaining:

    Article Title: Staphylococcal Nuclease and Tudor Domain Containing 1 (SND1 Protein) Promotes Hepatocarcinogenesis by Inhibiting Monoglyceride Lipase (MGLL)
    Article Snippet: .. Tissue Microarray and Immunostaining Human HCC tissue microarrays were obtained from Imgenex Corp. Two tissue microarrays were used as follows: one containing 40 primary HCC, 10 metastatic HCC, and 9 normal adjacent liver samples (Imgenex; IMH-360); and the other containing 46 primary HCC and 13 metastatic HCC (Imgenex; IMH-318). .. Immunostaining was performed using anti-SND1 antibody (rabbit polyclonal; 1:100; Prestige Antibodies® powered by Atlas antibodies from Sigma), anti-MGLL antibody (goat polyclonal; 1:250; Abcam), and anti-PCNA antibody (mouse monoclonal; 1:300; Cell Signaling) as described ( 16 ).



    Similar Products

    tumor tissue microarray for human hcc  (Servicebio Inc)
    90
    Servicebio Inc tumor tissue microarray for human hcc
    Tumor Tissue Microarray For Human Hcc, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/tumor+tissue+microarray+for+human+hcc/pm36693491-48-9-1
    Average 90 stars, based on 1 article reviews
    tumor tissue microarray for human hcc - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    tissue microarrays of human hcc tumor tissues and adjacent tissues  (Anhui Medical University)
    90
    Anhui Medical University tissue microarrays of human hcc tumor tissues and adjacent tissues
    Tissue Microarrays Of Human Hcc Tumor Tissues And Adjacent Tissues, supplied by Anhui Medical University, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/hcc+tissues/pm36604718-58-3-24
    Average 90 stars, based on 1 article reviews
    tissue microarrays of human hcc tumor tissues and adjacent tissues - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human hcc tissue microarray  (Servicebio Inc)
    90
    Servicebio Inc human hcc tissue microarray
    A. Pan-cancer analysis of YAP1 mRNA expression levels in different tumors by TIMER database. * P < 0.05 vs corresponding normal control group. B. The mRNA expression levels of YAP1 in <t>HCC</t> tumor (T, n=371) and normal tissues (N, n=50), and YAP1 expression in different HCC grades based on the TCGA database. * P < 0.05 vs the normal tissues. C. YAP1 expression in tumor (T) and para-tumor tissues (P) by the HCC tissue <t>microarray.</t> A 0, A17 and A18 indicate patient number. D. Analysis the relevance of the ratio of YAP1 (para-tumor /tumor tissues) with clinical tissue microarray.
    Human Hcc Tissue Microarray, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/tumor+tissue+microarray+for+human+hcc/bio_rxiv__2021__11__30__470572-202-0-7
    Average 90 stars, based on 1 article reviews
    human hcc tissue microarray - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human liver tissue microarray slides including a total of 76 normal, 38 hcc-nt and 115 hcc-t samples with 10 pairs of hcc-nt and hcc-t samples  (U.S Biomax Inc)
    90
    U.S Biomax Inc human liver tissue microarray slides including a total of 76 normal, 38 hcc-nt and 115 hcc-t samples with 10 pairs of hcc-nt and hcc-t samples
    USP2 protein expression levels were reduced <t>in</t> <t>HCC-T</t> tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.
    Human Liver Tissue Microarray Slides Including A Total Of 76 Normal, 38 Hcc Nt And 115 Hcc T Samples With 10 Pairs Of Hcc Nt And Hcc T Samples, supplied by U.S Biomax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/human+liver+tissue+microarray+slides+including+a+total+of+76+normal++38+hcc+nt+and+115+hcc+t+samples+with+10+pairs+of+hcc+nt+and+hcc+t+samples/pmc08569343-71-26-31
    Average 90 stars, based on 1 article reviews
    human liver tissue microarray slides including a total of 76 normal, 38 hcc-nt and 115 hcc-t samples with 10 pairs of hcc-nt and hcc-t samples - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human hcc tissue microarray tma  (Novus Biologicals)
    90
    Novus Biologicals human hcc tissue microarray tma
    Identification of downstream target genes commonly upregulated by mitochondrial defect and NFE2L1. Transcriptome data of four cell lines (Ch-L and SNU387 harboring active mitochondria, and SNU354 and SNU423 harboring defective mitochondria) were obtained from our previous report . Two independent sets of SNU387 cells transfected with pcDNA-NFE2L1 plasmid (NFE2L1_OE) for 48 h and of SNU423 cells with siNFE2L1 (NFE2L1_KD) for 72 h were applied to cDNA <t>microarray</t> for transcriptome profiling. ( A ) Venn diagram of commonly upregulated genes with mitochondrial defect (Mito defect, SNU354 and SNU423 vs. Ch-L and SNU387) and NFE2L1-dependent regulation. ( B ) Heatmaps show the expression of 10 commonly upregulated genes in four hepatoma cell lines (left), NFE2L1-depleted SNU423 cells (middle), and NFE2L1-overexpressed SNU387 cells (right). Each column and row represent independent samples and the indicated genes, respectively. Red and blue color indicates the high and low expression, respectively. ( C , E ) Validation of four target mRNA levels by qRT-PCR. ( D , F ) Validation of four target protein levels by Western blot analysis. #1 and #2 indicate two different siRNAs for NFE2L1. ** p < 0.01 vs. pcDNA or siNC by the Student t -test. ( G ) Associations of NFE2L1 with the 10 commonly upregulated genes were evaluated using the TCGA-LIHC cohort ( n = 371). Pearson’s product moment correlation test was performed. The correlation estimate and the p-value with statistical significance are marked. ( H ) Boxplot for STX12 expression level of primary <t>HCC</t> tissues from the TCGA-LIHC cohort. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. For comparison, primary tumors were divided into two groups, based on the median expression level of NFE2L1 and NDUFA9: a group with both above the median NFE2L1 level and below the median NDUFA9 level, a group with both below the median NFE2L1 level and above the median NDUFA9 level. p -value from Wilcoxon t-test is indicated.
    Human Hcc Tissue Microarray Tma, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/Human+Liver+Tissue+MicroArray+(Cancer+metastasis)/pmc07565734-219-5-11
    Average 90 stars, based on 1 article reviews
    human hcc tissue microarray tma - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    the tissue microarray of human hcc (bc03119)  (U.S Biomax Inc)
    90
    U.S Biomax Inc the tissue microarray of human hcc (bc03119)
    EIF3A expression was increased in HCC tissues of tumor model mice as well as patients with HCC compared to normal subjects. ( A ) The expression of EIF3A in liver tissues of H- ras 12V-Tg mice. Blots were probed with commercial anti-EIF3A antibody and XC90. Band intensities were quantified by Image J software and the values were normalized to β-actin. ( B ) Immunohistochemical analysis of EIF3A in liver tissues of HCC model mice. Liver tissues from wild type control mice (Non-Tg: WT) were also stained. NT: non-tumor, T: tumor region, H- ras 12V-Tg (n = 2), Non-Tg (n = 4), HBX-Tg-nonT: HBX-transgenic mouse without tumor (n = 3), HBX-Tg-ST: HBX-transgenic mouse with small tumor (n = 2), HBX-Tg-LT: HBX-transgenic mouse with large tumor (n = 3). Representative images were shown (all staining images were shown in Supplementary Fig. ). DAB intensity of each image was quantified using Image J. ( C ) Gene expression analysis of EIF3A in human tumor tissues using GENT. ( D ) Immunohistochemical staining of human liver tissues <t>microarray</t> with anti-EIF3A antibody (Normal liver = 10, liver cancer = 110 cases). Representative images were shown (all staining images were shown in Supplementary Fig. ). Statistical significance was determined by two-tailed Student’s t-test. ( E ) EIF3A in exosomes purified from hepatoma cell cultured media (HepG2 and Hepa-1c1c7 cells) analyzed by Western blotting. A well-known marker of the exosomal fraction, ALIX, was probed as a control.
    The Tissue Microarray Of Human Hcc (Bc03119), supplied by U.S Biomax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/the+tissue+microarray+of+human+hcc++bc03119+/pmc06667438-265-1-10
    Average 90 stars, based on 1 article reviews
    the tissue microarray of human hcc (bc03119) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    paraffin-embedded human hcc tissue microarrays csa4  (SuperBioChips)
    90
    SuperBioChips paraffin-embedded human hcc tissue microarrays csa4
    EIF3A expression was increased in HCC tissues of tumor model mice as well as patients with HCC compared to normal subjects. ( A ) The expression of EIF3A in liver tissues of H- ras 12V-Tg mice. Blots were probed with commercial anti-EIF3A antibody and XC90. Band intensities were quantified by Image J software and the values were normalized to β-actin. ( B ) Immunohistochemical analysis of EIF3A in liver tissues of HCC model mice. Liver tissues from wild type control mice (Non-Tg: WT) were also stained. NT: non-tumor, T: tumor region, H- ras 12V-Tg (n = 2), Non-Tg (n = 4), HBX-Tg-nonT: HBX-transgenic mouse without tumor (n = 3), HBX-Tg-ST: HBX-transgenic mouse with small tumor (n = 2), HBX-Tg-LT: HBX-transgenic mouse with large tumor (n = 3). Representative images were shown (all staining images were shown in Supplementary Fig. ). DAB intensity of each image was quantified using Image J. ( C ) Gene expression analysis of EIF3A in human tumor tissues using GENT. ( D ) Immunohistochemical staining of human liver tissues <t>microarray</t> with anti-EIF3A antibody (Normal liver = 10, liver cancer = 110 cases). Representative images were shown (all staining images were shown in Supplementary Fig. ). Statistical significance was determined by two-tailed Student’s t-test. ( E ) EIF3A in exosomes purified from hepatoma cell cultured media (HepG2 and Hepa-1c1c7 cells) analyzed by Western blotting. A well-known marker of the exosomal fraction, ALIX, was probed as a control.
    Paraffin Embedded Human Hcc Tissue Microarrays Csa4, supplied by SuperBioChips, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/human+tissue+arrays+containing+hcc+cells+and+corresponding+adjacent+non+cancerous+liver+tissues+csa4/pmc06405965-66-3-18
    Average 90 stars, based on 1 article reviews
    paraffin-embedded human hcc tissue microarrays csa4 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human hcc tissue microarrays cs4  (SuperBioChips)
    90
    SuperBioChips human hcc tissue microarrays cs4
    EIF3A expression was increased in HCC tissues of tumor model mice as well as patients with HCC compared to normal subjects. ( A ) The expression of EIF3A in liver tissues of H- ras 12V-Tg mice. Blots were probed with commercial anti-EIF3A antibody and XC90. Band intensities were quantified by Image J software and the values were normalized to β-actin. ( B ) Immunohistochemical analysis of EIF3A in liver tissues of HCC model mice. Liver tissues from wild type control mice (Non-Tg: WT) were also stained. NT: non-tumor, T: tumor region, H- ras 12V-Tg (n = 2), Non-Tg (n = 4), HBX-Tg-nonT: HBX-transgenic mouse without tumor (n = 3), HBX-Tg-ST: HBX-transgenic mouse with small tumor (n = 2), HBX-Tg-LT: HBX-transgenic mouse with large tumor (n = 3). Representative images were shown (all staining images were shown in Supplementary Fig. ). DAB intensity of each image was quantified using Image J. ( C ) Gene expression analysis of EIF3A in human tumor tissues using GENT. ( D ) Immunohistochemical staining of human liver tissues <t>microarray</t> with anti-EIF3A antibody (Normal liver = 10, liver cancer = 110 cases). Representative images were shown (all staining images were shown in Supplementary Fig. ). Statistical significance was determined by two-tailed Student’s t-test. ( E ) EIF3A in exosomes purified from hepatoma cell cultured media (HepG2 and Hepa-1c1c7 cells) analyzed by Western blotting. A well-known marker of the exosomal fraction, ALIX, was probed as a control.
    Human Hcc Tissue Microarrays Cs4, supplied by SuperBioChips, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/human+hepatocellular+carcinoma+tissue+arrays/pm28652022-56-1-16
    Average 90 stars, based on 1 article reviews
    human hcc tissue microarrays cs4 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human hcc and nsclc tissue microarrays  (Novus Biologicals)
    90
    Novus Biologicals human hcc and nsclc tissue microarrays
    EIF3A expression was increased in HCC tissues of tumor model mice as well as patients with HCC compared to normal subjects. ( A ) The expression of EIF3A in liver tissues of H- ras 12V-Tg mice. Blots were probed with commercial anti-EIF3A antibody and XC90. Band intensities were quantified by Image J software and the values were normalized to β-actin. ( B ) Immunohistochemical analysis of EIF3A in liver tissues of HCC model mice. Liver tissues from wild type control mice (Non-Tg: WT) were also stained. NT: non-tumor, T: tumor region, H- ras 12V-Tg (n = 2), Non-Tg (n = 4), HBX-Tg-nonT: HBX-transgenic mouse without tumor (n = 3), HBX-Tg-ST: HBX-transgenic mouse with small tumor (n = 2), HBX-Tg-LT: HBX-transgenic mouse with large tumor (n = 3). Representative images were shown (all staining images were shown in Supplementary Fig. ). DAB intensity of each image was quantified using Image J. ( C ) Gene expression analysis of EIF3A in human tumor tissues using GENT. ( D ) Immunohistochemical staining of human liver tissues <t>microarray</t> with anti-EIF3A antibody (Normal liver = 10, liver cancer = 110 cases). Representative images were shown (all staining images were shown in Supplementary Fig. ). Statistical significance was determined by two-tailed Student’s t-test. ( E ) EIF3A in exosomes purified from hepatoma cell cultured media (HepG2 and Hepa-1c1c7 cells) analyzed by Western blotting. A well-known marker of the exosomal fraction, ALIX, was probed as a control.
    Human Hcc And Nsclc Tissue Microarrays, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+tissue+microarrays/human+hcc+tissue+microarrays/pmc05130016-232-1-10
    Average 90 stars, based on 1 article reviews
    human hcc and nsclc tissue microarrays - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Pan-cancer analysis of YAP1 mRNA expression levels in different tumors by TIMER database. * P < 0.05 vs corresponding normal control group. B. The mRNA expression levels of YAP1 in HCC tumor (T, n=371) and normal tissues (N, n=50), and YAP1 expression in different HCC grades based on the TCGA database. * P < 0.05 vs the normal tissues. C. YAP1 expression in tumor (T) and para-tumor tissues (P) by the HCC tissue microarray. A 0, A17 and A18 indicate patient number. D. Analysis the relevance of the ratio of YAP1 (para-tumor /tumor tissues) with clinical tissue microarray.

    Journal: bioRxiv

    Article Title: Dihydroartemisinin broke immune evasion through YAP1/JAK1/STAT1, 3 pathways to enhance anti-PD-1 therapy in hepatocellular carcinoma

    doi: 10.1101/2021.11.30.470572

    Figure Lengend Snippet: A. Pan-cancer analysis of YAP1 mRNA expression levels in different tumors by TIMER database. * P < 0.05 vs corresponding normal control group. B. The mRNA expression levels of YAP1 in HCC tumor (T, n=371) and normal tissues (N, n=50), and YAP1 expression in different HCC grades based on the TCGA database. * P < 0.05 vs the normal tissues. C. YAP1 expression in tumor (T) and para-tumor tissues (P) by the HCC tissue microarray. A 0, A17 and A18 indicate patient number. D. Analysis the relevance of the ratio of YAP1 (para-tumor /tumor tissues) with clinical tissue microarray.

    Article Snippet: Human HCC tissue microarray was purchased from Servicebio (China, Wuhan, no: IWLT-N-64LV41 Live C-1401).

    Techniques: Expressing, Control, Microarray

    The expression of YAP1 in tumor (T) and para-tumor tissues (P) by the HCC tissue microarray. Axx indicate patient number.

    Journal: bioRxiv

    Article Title: Dihydroartemisinin broke immune evasion through YAP1/JAK1/STAT1, 3 pathways to enhance anti-PD-1 therapy in hepatocellular carcinoma

    doi: 10.1101/2021.11.30.470572

    Figure Lengend Snippet: The expression of YAP1 in tumor (T) and para-tumor tissues (P) by the HCC tissue microarray. Axx indicate patient number.

    Article Snippet: Human HCC tissue microarray was purchased from Servicebio (China, Wuhan, no: IWLT-N-64LV41 Live C-1401).

    Techniques: Expressing, Microarray

    Journal: bioRxiv

    Article Title: Dihydroartemisinin broke immune evasion through YAP1/JAK1/STAT1, 3 pathways to enhance anti-PD-1 therapy in hepatocellular carcinoma

    doi: 10.1101/2021.11.30.470572

    Figure Lengend Snippet:

    Article Snippet: Human HCC tissue microarray was purchased from Servicebio (China, Wuhan, no: IWLT-N-64LV41 Live C-1401).

    Techniques: Expressing, Microarray

    USP2 protein expression levels were reduced in HCC-T tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: USP2 protein expression levels were reduced in HCC-T tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing, Staining

    USP2 mRNA expression levels were decreased in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (C) individual pairs (n=8). *P<0.05 and **P<0.01 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: USP2 mRNA expression levels were decreased in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (C) individual pairs (n=8). *P<0.05 and **P<0.01 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Dysregulation of Usp2 mRNA expression in HCC-T tissues from HCC mice. A. The expression levels of Usp2 mRNA in mouse normal liver (n=16), HCC-NT (n=15) and HCC-T (n=15) samples. B. The expression levels of USP2 mRNA in paired HCC-T and HCC-NT tissues. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: Dysregulation of Usp2 mRNA expression in HCC-T tissues from HCC mice. A. The expression levels of Usp2 mRNA in mouse normal liver (n=16), HCC-NT (n=15) and HCC-T (n=15) samples. B. The expression levels of USP2 mRNA in paired HCC-T and HCC-NT tissues. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing

    USP2b mRNA expression levels were markedly reduced in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) USP2b protein detected by Western blot in normal liver (n=8), HCC-NT (n=8) and HCC-T tissues (n=8). (C) Quantification of the USP2b protein levels in (B) by ImageJ. (D) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs (n=8). ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: USP2b mRNA expression levels were markedly reduced in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) USP2b protein detected by Western blot in normal liver (n=8), HCC-NT (n=8) and HCC-T tissues (n=8). (C) Quantification of the USP2b protein levels in (B) by ImageJ. (D) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs (n=8). ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    Usp2b mRNA expression levels were significantly deceased in HCC-T tissues from HCC mice. (A) The expression levels of Usp2b mRNA in mouse normal liver (n=16), HCC-NT (n=26) and HCC-T (n=26) samples. (B) The expression levels of Usp2b mRNA in paired HCC-T and HCC-NT tissues (n=26). (C) Representative Western blots of Usp2b protein in normal liver (n=18), HCC-NT (n=18) and HCC-T tissues (n=17). (D) Quantification of Usp2b protein in (C) by ImageJ. *P<0.05, **P<0.01 and ***P<0.001 in one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: Usp2b mRNA expression levels were significantly deceased in HCC-T tissues from HCC mice. (A) The expression levels of Usp2b mRNA in mouse normal liver (n=16), HCC-NT (n=26) and HCC-T (n=26) samples. (B) The expression levels of Usp2b mRNA in paired HCC-T and HCC-NT tissues (n=26). (C) Representative Western blots of Usp2b protein in normal liver (n=18), HCC-NT (n=18) and HCC-T tissues (n=17). (D) Quantification of Usp2b protein in (C) by ImageJ. *P<0.05, **P<0.01 and ***P<0.001 in one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing, Western Blot

    Identification of downstream target genes commonly upregulated by mitochondrial defect and NFE2L1. Transcriptome data of four cell lines (Ch-L and SNU387 harboring active mitochondria, and SNU354 and SNU423 harboring defective mitochondria) were obtained from our previous report . Two independent sets of SNU387 cells transfected with pcDNA-NFE2L1 plasmid (NFE2L1_OE) for 48 h and of SNU423 cells with siNFE2L1 (NFE2L1_KD) for 72 h were applied to cDNA microarray for transcriptome profiling. ( A ) Venn diagram of commonly upregulated genes with mitochondrial defect (Mito defect, SNU354 and SNU423 vs. Ch-L and SNU387) and NFE2L1-dependent regulation. ( B ) Heatmaps show the expression of 10 commonly upregulated genes in four hepatoma cell lines (left), NFE2L1-depleted SNU423 cells (middle), and NFE2L1-overexpressed SNU387 cells (right). Each column and row represent independent samples and the indicated genes, respectively. Red and blue color indicates the high and low expression, respectively. ( C , E ) Validation of four target mRNA levels by qRT-PCR. ( D , F ) Validation of four target protein levels by Western blot analysis. #1 and #2 indicate two different siRNAs for NFE2L1. ** p < 0.01 vs. pcDNA or siNC by the Student t -test. ( G ) Associations of NFE2L1 with the 10 commonly upregulated genes were evaluated using the TCGA-LIHC cohort ( n = 371). Pearson’s product moment correlation test was performed. The correlation estimate and the p-value with statistical significance are marked. ( H ) Boxplot for STX12 expression level of primary HCC tissues from the TCGA-LIHC cohort. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. For comparison, primary tumors were divided into two groups, based on the median expression level of NFE2L1 and NDUFA9: a group with both above the median NFE2L1 level and below the median NDUFA9 level, a group with both below the median NFE2L1 level and above the median NDUFA9 level. p -value from Wilcoxon t-test is indicated.

    Journal: Cancers

    Article Title: Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis

    doi: 10.3390/cancers12092632

    Figure Lengend Snippet: Identification of downstream target genes commonly upregulated by mitochondrial defect and NFE2L1. Transcriptome data of four cell lines (Ch-L and SNU387 harboring active mitochondria, and SNU354 and SNU423 harboring defective mitochondria) were obtained from our previous report . Two independent sets of SNU387 cells transfected with pcDNA-NFE2L1 plasmid (NFE2L1_OE) for 48 h and of SNU423 cells with siNFE2L1 (NFE2L1_KD) for 72 h were applied to cDNA microarray for transcriptome profiling. ( A ) Venn diagram of commonly upregulated genes with mitochondrial defect (Mito defect, SNU354 and SNU423 vs. Ch-L and SNU387) and NFE2L1-dependent regulation. ( B ) Heatmaps show the expression of 10 commonly upregulated genes in four hepatoma cell lines (left), NFE2L1-depleted SNU423 cells (middle), and NFE2L1-overexpressed SNU387 cells (right). Each column and row represent independent samples and the indicated genes, respectively. Red and blue color indicates the high and low expression, respectively. ( C , E ) Validation of four target mRNA levels by qRT-PCR. ( D , F ) Validation of four target protein levels by Western blot analysis. #1 and #2 indicate two different siRNAs for NFE2L1. ** p < 0.01 vs. pcDNA or siNC by the Student t -test. ( G ) Associations of NFE2L1 with the 10 commonly upregulated genes were evaluated using the TCGA-LIHC cohort ( n = 371). Pearson’s product moment correlation test was performed. The correlation estimate and the p-value with statistical significance are marked. ( H ) Boxplot for STX12 expression level of primary HCC tissues from the TCGA-LIHC cohort. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. For comparison, primary tumors were divided into two groups, based on the median expression level of NFE2L1 and NDUFA9: a group with both above the median NFE2L1 level and below the median NDUFA9 level, a group with both below the median NFE2L1 level and above the median NDUFA9 level. p -value from Wilcoxon t-test is indicated.

    Article Snippet: Briefly, paraffin-embedded tissue slides of human HCC tissue microarray (TMA) (NBP2-30221, Novus Biologicals) were deparaffinized and rehydrated.

    Techniques: Transfection, Plasmid Preparation, Microarray, Expressing, Biomarker Discovery, Quantitative RT-PCR, Western Blot, Comparison

    STX12 is a key regulator of hepatoma cell invasiveness. ( A , B ) SNU423 cell was transfected with STX12 siRNA for 72 h. ( A ) Cell invasion assay using a Matrigel-coated Transwell system. Representative invaded cell images are shown in the right panel. ** p < 0.01 vs. siNC by the Student t-test. ( B ) Western blot (left) and cell growth rate (right). #1 and #2 indicate two different siRNAs for STX12. ( C , D ) Ch-L was transfected with STX12 plasmid for 48 h. ( C ) Cell invasion assay. Representative invaded cell images are shown in the right panel. ** p < 0.01 vs. GFP by the Student t-test. ( D ) Western blot (left) and cell growth rate (right). ( E – G ) SNU423 clone, which was stably suppressing NFE2L1 with sgNFE2L1 RNA, was further infected with lentivirus harboring STX12 or GFP. ( E ) Cell invasion assay. ** p < 0.01 vs. sgNC; # < 0.05 vs. GFP by the Student t -test. ( F ) Cell growth. ( G ) Western blot. ( H ) Immunohistochemistry of HCC tissue microarray, as described in ‘Materials and Methods’. Lower panel shows representative images for NFE2L1 and STX12 immunostaining. ( I , J ) Boxplots of the normalized enrichment score (NES) for EMT signature of the primary HCC tissues from the TCGA_LIHC dataset. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. p -values from Welch two-sample t -test are indicated. ( I ) Tumor samples were divided into high and low groups, according to the expression level of NFE2L1 (left) or STX12 (middle), respectively, as described in ‘Materials and Methods’. To evaluate the effect of co-expression on the EMT signature, tumor samples were divided into two groups, based on the co-expression level of both NFE2L1 and STX12 (right). ( J ) The NFE2L1 and STX12 co-expressing group (right panel of I) was further subdivided into an H ( n = 11) and L group ( n = 23) according to the NDUFA9 expression level. ( K ) Overall survival (OS) time of the H and L group was compared based on the Kaplan–Meier survival analysis. Statistical significance for KM survival was estimated by the Cox–Mantel log-rank test.

    Journal: Cancers

    Article Title: Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis

    doi: 10.3390/cancers12092632

    Figure Lengend Snippet: STX12 is a key regulator of hepatoma cell invasiveness. ( A , B ) SNU423 cell was transfected with STX12 siRNA for 72 h. ( A ) Cell invasion assay using a Matrigel-coated Transwell system. Representative invaded cell images are shown in the right panel. ** p < 0.01 vs. siNC by the Student t-test. ( B ) Western blot (left) and cell growth rate (right). #1 and #2 indicate two different siRNAs for STX12. ( C , D ) Ch-L was transfected with STX12 plasmid for 48 h. ( C ) Cell invasion assay. Representative invaded cell images are shown in the right panel. ** p < 0.01 vs. GFP by the Student t-test. ( D ) Western blot (left) and cell growth rate (right). ( E – G ) SNU423 clone, which was stably suppressing NFE2L1 with sgNFE2L1 RNA, was further infected with lentivirus harboring STX12 or GFP. ( E ) Cell invasion assay. ** p < 0.01 vs. sgNC; # < 0.05 vs. GFP by the Student t -test. ( F ) Cell growth. ( G ) Western blot. ( H ) Immunohistochemistry of HCC tissue microarray, as described in ‘Materials and Methods’. Lower panel shows representative images for NFE2L1 and STX12 immunostaining. ( I , J ) Boxplots of the normalized enrichment score (NES) for EMT signature of the primary HCC tissues from the TCGA_LIHC dataset. Bottom, middle, and top lines of each box indicate the first quartile, median, and third quartile values, respectively. Whiskers represent the minimum and maximum values. p -values from Welch two-sample t -test are indicated. ( I ) Tumor samples were divided into high and low groups, according to the expression level of NFE2L1 (left) or STX12 (middle), respectively, as described in ‘Materials and Methods’. To evaluate the effect of co-expression on the EMT signature, tumor samples were divided into two groups, based on the co-expression level of both NFE2L1 and STX12 (right). ( J ) The NFE2L1 and STX12 co-expressing group (right panel of I) was further subdivided into an H ( n = 11) and L group ( n = 23) according to the NDUFA9 expression level. ( K ) Overall survival (OS) time of the H and L group was compared based on the Kaplan–Meier survival analysis. Statistical significance for KM survival was estimated by the Cox–Mantel log-rank test.

    Article Snippet: Briefly, paraffin-embedded tissue slides of human HCC tissue microarray (TMA) (NBP2-30221, Novus Biologicals) were deparaffinized and rehydrated.

    Techniques: Transfection, Invasion Assay, Western Blot, Plasmid Preparation, Stable Transfection, Infection, Immunohistochemistry, Microarray, Immunostaining, Expressing

    EIF3A expression was increased in HCC tissues of tumor model mice as well as patients with HCC compared to normal subjects. ( A ) The expression of EIF3A in liver tissues of H- ras 12V-Tg mice. Blots were probed with commercial anti-EIF3A antibody and XC90. Band intensities were quantified by Image J software and the values were normalized to β-actin. ( B ) Immunohistochemical analysis of EIF3A in liver tissues of HCC model mice. Liver tissues from wild type control mice (Non-Tg: WT) were also stained. NT: non-tumor, T: tumor region, H- ras 12V-Tg (n = 2), Non-Tg (n = 4), HBX-Tg-nonT: HBX-transgenic mouse without tumor (n = 3), HBX-Tg-ST: HBX-transgenic mouse with small tumor (n = 2), HBX-Tg-LT: HBX-transgenic mouse with large tumor (n = 3). Representative images were shown (all staining images were shown in Supplementary Fig. ). DAB intensity of each image was quantified using Image J. ( C ) Gene expression analysis of EIF3A in human tumor tissues using GENT. ( D ) Immunohistochemical staining of human liver tissues microarray with anti-EIF3A antibody (Normal liver = 10, liver cancer = 110 cases). Representative images were shown (all staining images were shown in Supplementary Fig. ). Statistical significance was determined by two-tailed Student’s t-test. ( E ) EIF3A in exosomes purified from hepatoma cell cultured media (HepG2 and Hepa-1c1c7 cells) analyzed by Western blotting. A well-known marker of the exosomal fraction, ALIX, was probed as a control.

    Journal: Scientific Reports

    Article Title: Serum anti-EIF3A autoantibody as a potential diagnostic marker for hepatocellular carcinoma

    doi: 10.1038/s41598-019-47365-4

    Figure Lengend Snippet: EIF3A expression was increased in HCC tissues of tumor model mice as well as patients with HCC compared to normal subjects. ( A ) The expression of EIF3A in liver tissues of H- ras 12V-Tg mice. Blots were probed with commercial anti-EIF3A antibody and XC90. Band intensities were quantified by Image J software and the values were normalized to β-actin. ( B ) Immunohistochemical analysis of EIF3A in liver tissues of HCC model mice. Liver tissues from wild type control mice (Non-Tg: WT) were also stained. NT: non-tumor, T: tumor region, H- ras 12V-Tg (n = 2), Non-Tg (n = 4), HBX-Tg-nonT: HBX-transgenic mouse without tumor (n = 3), HBX-Tg-ST: HBX-transgenic mouse with small tumor (n = 2), HBX-Tg-LT: HBX-transgenic mouse with large tumor (n = 3). Representative images were shown (all staining images were shown in Supplementary Fig. ). DAB intensity of each image was quantified using Image J. ( C ) Gene expression analysis of EIF3A in human tumor tissues using GENT. ( D ) Immunohistochemical staining of human liver tissues microarray with anti-EIF3A antibody (Normal liver = 10, liver cancer = 110 cases). Representative images were shown (all staining images were shown in Supplementary Fig. ). Statistical significance was determined by two-tailed Student’s t-test. ( E ) EIF3A in exosomes purified from hepatoma cell cultured media (HepG2 and Hepa-1c1c7 cells) analyzed by Western blotting. A well-known marker of the exosomal fraction, ALIX, was probed as a control.

    Article Snippet: The tissue microarray of human HCC (BC03119) was purchased from US Biomax (Rockville, MD, USA).

    Techniques: Expressing, Software, Immunohistochemical staining, Staining, Transgenic Assay, Microarray, Two Tailed Test, Purification, Cell Culture, Western Blot, Marker